Figure 3

Analysis of furrow ingression and multinucleation. (A) HeLa cells expressing the indicated RLC construct were imaged throughout cell division. Furrow width was measured every 30 seconds for a minimum of 5 cells. Data are mean +/- SEM. * indicates p = <0.05. (B) HeLa cells expressing the indicated RLC construct or treated with 60 uM blebbistatin for 24 hours were analyzed for nucleation state using propidium iodide and flow cytometry. Black bars represent cells with DNA content of less than 4n. Grey bars represent cells with DNA content of 4n or greater.