Figure 4
Pluripotency of bioreactor-differentiated embryoid bodies. A) Differentiated ESCs retained their ability to form ESC-like colonies upon plating on gelatin-coated culture plates. The morphology of the colonies as well as expression of pluripotency markers showed the pluripotency of differentiated cells after 25 days in suspension bioreactor without drug selection. Immunostaining was performed with antibodies against Nanog and Oct4. B) Confocal microscopy of day 25 EBs showed the simultaneous expression of α-MHC and Oct4 within the bioreactor-derived aggregates. Scale bar: 100 μm.