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Table 1 Primer sequences used in RT-PCR and Quantitative-PCR

From: Impact of stirred suspension bioreactor culture on the differentiation of murine embryonic stem cells into cardiomyocytes

Gene Sequences Product Size Indication
Oct4 Forward: 5'-GGCGTTCTCTTTGGAAAGGTGTTC-3'
Reverse: 5'-CTGGAACCACATCCTTCTCT-3'
312bp Pluripotency
Sox2 Forward: 5'-CACAACTCGGAGATCAGCAA-3'
Reverse: 5'-CTCCGGGAAGCGTGTACTTA-3'
190bp Pluripotency
Nanog Forward: 5'-AAGCAGAAGATGCGGACTGT-3'
Reverse: 5'-GTGCTGAGCCCTTCTGAATC-3'
232bp Pluripotency
α-MHC Forward: 5'-CTGCTGGAGAGGTTATTCCTCG-3'
Reverse: 5'-GGAAGAGTGAGCGGCGCATCAAGG-3'
301bp Cardiomyocyte
ALCAM Forward:5'-CTTGCACAGCAGAAAACCAA-3'
Reverse: 5'-TAGACGACACCAGCAACGAG-3'
190bp Cardiogenesis
PCAM-1 Forward: 5'-TGCAGGAGTCCTTCTCCACT-3'
Reverse: 5'-ACGGTTTGATTCCACTTTGC-3'
245bp Early mesoderm
ANF Forward: 5'-TGATAGATGAAGGCAGGAAGCCGC-3'
Reverse:5'-GGATTGGAGCCCAGAGTGGACTAGG-3'
203bp Cardiomyocyte
GAPDH Forward: 5'-AACTTTGGCATTGTGGAAGG-3'
Reverse: 5'-ACACATTGGGGGTAGGAACA-3'
223bp Housekeeping gene