Table 1 Primer sequences used in RT-PCR and Quantitative-PCR

Oct4

Forward: 5'-GGCGTTCTCTTTGGAAAGGTGTTC-3'

Reverse: 5'-CTGGAACCACATCCTTCTCT-3'

Pluripotency

Sox2

Forward: 5'-CACAACTCGGAGATCAGCAA-3'

Reverse: 5'-CTCCGGGAAGCGTGTACTTA-3'

Pluripotency

Nanog

Forward: 5'-AAGCAGAAGATGCGGACTGT-3'

Reverse: 5'-GTGCTGAGCCCTTCTGAATC-3'

Pluripotency

α-MHC

Forward: 5'-CTGCTGGAGAGGTTATTCCTCG-3'

Reverse: 5'-GGAAGAGTGAGCGGCGCATCAAGG-3'

Cardiomyocyte

ALCAM

Forward:5'-CTTGCACAGCAGAAAACCAA-3'

Reverse: 5'-TAGACGACACCAGCAACGAG-3'

Cardiogenesis

PCAM-1

Forward: 5'-TGCAGGAGTCCTTCTCCACT-3'

Reverse: 5'-ACGGTTTGATTCCACTTTGC-3'

Early mesoderm

ANF

Forward: 5'-TGATAGATGAAGGCAGGAAGCCGC-3'

Reverse:5'-GGATTGGAGCCCAGAGTGGACTAGG-3'

Cardiomyocyte

GAPDH

Forward: 5'-AACTTTGGCATTGTGGAAGG-3'

Reverse: 5'-ACACATTGGGGGTAGGAACA-3'

Housekeeping gene