Figure 1
Effect of JNK inhibition on cell death and it's mediators in hyperoxia-exposed cells. A549 cells were exposed to 21% up to 95% O2 and cell viability (trypan blue) was assessed at 24 h (1A). MLE-12 cells were exposed to 21% up to 95% O2 and cell viability (TUNEL assay) was assessed at 24 h (1B). The noted values represent assessments in a minimum of 4 measurements in each group. Next, A549 cells were exposed to 21% up to 95% O2 and FAS, FAS-L, procaspase 3 and cleaved caspase 3 proteins were assessed by Western Blot. Independent experiments were done in the presence of the JNK pathway inhibitor. The ratios of FAS-L and cleaved caspase-3 with β-actin were quantified by densitometery (1C and 1D). The figures are illustrative of a minimum of 4 experiments. O2: oxygen; JNKi: JNK inhibitor. *P < 0.05, **P ≤ 0.02, #P ≤ 0.01.