Table 2 Timing of apoptotic events in the ovulated Xenopus eggs and in vitro-matured oocytes.
From: Unfertilized frog eggs die by apoptosis following meiotic exit
Events in eggs | Control eggs | PG-treated oocytes | Ionophore- activated eggs | Roscovitine- treated eggs |
|---|---|---|---|---|
Meiotic exit | within 18 h | within 24 h | within 45 min | within 6 h |
Cytochrome c release | 24 h | 24-36 h | 18 h | ND* |
Caspase 3/7 activation | 24-36 h | 48-60 h | 18-24 h | 18-36 h |
ATP decrease | 30 h | 60 h | 24 h | 24 h |
ADP/ATP increase | 30 h | 60 h | 24 h | 24 h |
Drop in intracellular pH (< 6.0) | 36 h | 60 h | 36 h | ND* |
Increase in egg diameter | 36 h | 60 h | 36 h | 24 h |