Colocalization of AnxA6 with a-actinin in cardiomyocytes. The representative images shown are endogenous expression of AnxA6 (a) and α-actinin (b) in the same cell and their colocalization pattern (c). The line profile for quantification of colocalization (d) was done using the merged image (inset) of AnxA6 and sarcomeric α-actinin. The images represent results of three different experiments with separate batches of myocyte preparation. Scale bar represents 10 μm (100×).