Table 2 Fission yeast strains used in this study
From: Auxin-inducible protein depletion system in fission yeast
Strain name | Genotype | Source |
|---|---|---|
972 | h - | Lab stock |
TNF47 | h - ade6X ura4-D18 | Lab stock |
HM1813 | h - ade6::ade6 + -P nmt41 -AtTIR1-9myc ura4-D18 | This work |
HM1905 | h - ade6::ade6 + -P nmt41 -AtTIR1-9myc mcm4::mcm4-2HA-IAA17 | This work |
HM1909 | h - mcm4::mcm4-2HA-IAA17 | This work |
HM1910 | h + mcm4::mcm4-2HA-IAA17 leu1-32 | This work |
HM2423 | h - mcm4::mcm4-2HA-IAA17 ade6X | This work |
HM2468 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc ura4-D18 | This work |
HM2473 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc mcm4::mcm4-2HA-IAA17 | This work |
HM2475 | h - ade6::ade6 + -P adh81 -skp1-AtTIR1-2NLS-9myc mcm4::mcm4-2HA-IAA17 | This work |
HM2491 | h - ade6::ade6 + -P nmt41 -skp1-AtTIR1-2NLS-9myc mcm4::mcm4-2HA-IAA17 | This work |
HM2550 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc mcm10::mcm10-IAA17 | This work |
HM2551 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc cdc20::cdc20-IAA17 | This work |
HM2552 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc pol1::pol1-IAA17 | This work |
HM2572 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc orc2::orc2-IAA17 | This work |
HM2575 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc orc6::orc6-IAA17 | This work |
HM2578 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc cdc45::cdc45-IAA17 | This work |
HM2580 | h - cdc45::cdc45-IAA17 | This work |
HM2985 | h - ade6::ade6 + -P adh15 -skp1-OsTIR1-natMX6-P adh15 -skp1-AtTIR1-2NLS ura4-D18 | This work |
HM3086 | h - cdc25-22 ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS-9myc mcm4::mcm4-2HA-IAA17 | This work |
HM3150 | h - ade6::ade6 + -P adh15 -skp1-AtTIR1-2NLS pol1::hphMX6-P nmt81 -pol1-IAA17 | This work |
HM3325 | h - cdc45::hphMX6-P nmt81 -cdc45-IAA17 | This work |
HM3390 | h - cdc25-22 cdc45::hphMX6-P nmt81 -cdc45-IAA17 mcm10::6flag-mcm10 ade6::ade6 + -P adh15 -skp1-OsTIR1-natMX6-P adh15 -skp1-AtTIR1-2NLS | This work |
HM3397 | h - cdc25-22 pol1::hphMX6-P nmt81 -pol1-IAA17 mcm10::6flag-mcm10 ade6::ade6 + -P adh15 -skp1-OsTIR1-natMX6-P adh15 -skp1-AtTIR1-2NLS | This work |
MKF53 | h + mcm4::mcm4-2HA-IAA17 leu1-32 pREP41D | This work |
MKF54 | h + mcm4::mcm4-2HA-IAA17 leu1-32 pREP41D-AtTIR1-9myc | This work |
MKF55 | h + mcm4::mcm4-2HA-IAA17 leu1-32 pREP41D-AtTIR1-2NLS-9myc | This work |
MKF60 | h + mcm4::mcm4-2HA-IAA17 leu1-32 pREP41D-skp1-AtTIR1-9myc | This work |