Table 3 Primers used in this study
From: Auxin-inducible protein depletion system in fission yeast
Primer name | Sequence |
|---|---|
IAA17-2HA-R | 5'-AAAATGCATTGCTGCAGCTCGAGCTCTGCTCTTGCAC-3' |
IAA17-2HA-F | 5'-AAAATGCATGCTGCAGCTCGAGCATACCCATACGATGTACCTGATTATGC TGGTTATCCTTATGATGTTCCAGACTATGCTATGATGGGCAGTGTCGAG-3' |
mcm4C-F | 5'-GGAATTCCATATGACTAGTGCAACTGATCCTGCAACAGGA-3' |
mcm4C-R | 5'-TTCCCCCGGGTTATCGTCGACTACCTGCAGGATCAGTATGTGCAATTGAA CGTAC-3' |
mcm4dw-F | 5'-GGGGTACCGAATTCAGCGAATAAGCTGGTATTATTCATGAGC-3' |
mcm4dw-R2 | 5'-GGAAGATCTGGATCCTCGAGCTACGAATCATGGCGAATTATTGGTTACG-3' |
skp1-F | 5'-CCATCGATCATATGGCTAGCCCTGCAGGGATGTCCAAAATCAAACTGATT TCATCTGAC-3' |
skp1-R | 5'-GGAATTCACTAGTCAGATCTCCTGCGCCGGCTCCAGCACCAAGATCTGG GATCCCTCTGTCTTCGGCCC-3' |
cdc20-IAA | 5'-TCGACACTGCCCATCATAGCTGCGGAAGTTGAGTTCAGCACAGAAAGTA-3' |
cdc20-ura | 5'-GTTTCGTCAATATCACAAGCCTCGCCTGACCATGAGC-3' |
cdc20-dw-R | 5'-CGAACGTTTAAGAGCATG-3' |
cdc20C-F | 5'-GACATGGGGACCTTGGTG-3' |
mcm10-IAA | 5'-TCGACACTGCCCATCATAGCAGCGGAAGTTGAGGGAACTATTTCTAAGTC-3' |
mcm10-ura | 5'-GTTTCGTCAATATCACAAGCGACTTAGAAATAGTTCCC-3' |
mcm10-dw-R | 5'-GCTTACAAGCCCATCATACC-3' |
mcm10C-F | 5'-CCCTAAATCCTCTCTACC-3' |
cdc45-IAA | 5'-TCGACACTGCCCATCATCGTTCCTTGTGGTAGTGTTTTGAAGGACAGAC-3' |
cdc45-ura | 5'-GTTTCGTCAATATCACAAGCTCCACACCAGCAATTGTT-3' |
cdc45-dw-R | 5'-TGACTGATCCAGAATCGG-3' |
cdc45C-F | 5'-GCATCCCCTTGCGTTAAC-3' |
orc2-IAA | 5'-TCGACACTGCCCATCATCGTTCCTTGTGGTACGTCTTCCATCATATCC-3' |
orc2-ura | 5'-GTTTCGTCAATATCACAAGCGGACCCTTTTAGACTAGG-3' |
orc2-dw-R | 5'-TACAATGCTGATACTAGGA-3' |
orc2C-F | 5'-TCTCTATTCTTTGCCCGCC-3' |
orc6-IAA | 5'-TCGACACTGCCCATCATCGTTCCTTGTGGTGAAGCAGTACCATCTTTTTC-3' |
orc6-ura | 5'-GTTTCGTCAATATCACAAGCCGGGTACACGATATCTTTAG-3' |
orc6-dw-R | 5'-CAATGATTCTCAAGAGACG-3' |
orc6C-F | 5'-ACTAGTATCGGCAAAAGCTTTTG-3' |
pol1-IAA | 5'-TCGACACTGCCCATCATCGTTCCTTGTGGCGATGAAAATATCAGTCCC-3' |
pol1-ura | 5'-GTTTCGTCAATATCACAAGCCTACCCGTTTAAGTAATCTAC-3' |
pol1-dw-R | 5'-GTCAAACATGTAGTGAGTAC-3' |
pol1C-F | 5'-CTCATCTCAACTCAGAGA-3' |
TIR1C-BmSc | 5'-CGGGATCCGCGGAGACAGTGACTTAGGCAT-3' |
TIR1-NLS-R | 5'-CGGGATCCTTAGTCGACCACTTTGCGTTTTTTCTTTGG-3' |
pol1N-nmt-F | 5'-CTTATAGTCGCTTTGTTAAATCATATGAGAAAGAGAAACGCGGG-3' |
pol1up-F-Spe | 5'-GGACTAGTCGTGCTTCAAGTATTTCCCG-3' |
pol1up-R-Bgl | 5'-GAAGATCTGTTCACGAGAAGACTTTAAAG-3' |
pol1dw-R-Bm | 5'-CGGGATCCCGACCAGACGATCCTTTTTC-3' |
cdc45up-F-Spe | 5'-GGACTAGTCGTTGTGCACATGTTCACCT-3' |
cdc45up-R-Bm | 5'-CGGGATCCACGTTACTGGTGGTGGATCA-3' |
ars2004 region-273F | 5'-CGGATCCGTAATCCCACAAA-3' |
ars2004 region-338R | 5'-TTTGCTTACATTTTCGGGAACTTA-3' |
nonARS1 region-514F | 5'-TACGCGACGAACCTTGCATAT-3' |
nonARS1 region-583R | 5'-TTATCAGACCATGGAGCCCAT-3' |