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Figure 1 | BMC Cell Biology

Figure 1

From: Depletion of the actin bundling protein SM22/transgelin increases actin dynamics and enhances the tumourigenic phenotypes of cells

Figure 1

Expression and localization of transgelin family proteins in REF52 cells. A, western blot analysis of SM22, NP25 and NP22 levels in various cell lines as indicated (S3T3 = Swiss 3T3). REF52 cells were chosen for further analysis due to their amenable phenotype and the expression of only SM22α. B-G, REF52 cells stained with Alexa 488 phalloidin (red in merge) to reveal F-actin containing structures (B, E), and stained for endogenous SM22α (C), or expressing SM22α-GFP (F) green in merges. Merged images (D, G) all show strong co-localization between endogenous and exogenous SM22 proteins and F-actin stress fibres. Nucleus counterstained with DAPI (blue).

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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