Figure 3

Effect of SM22α depletion on cell motility. Stable depletion of SM22α by expressing shRNA achieved 57% knockdown in REF52 cell Clone 1B, compared to cells expressing a control sense RNA hairpin (A). Cell migration was inhibited in SM22 knockdown cells, single cell trajectories were reduced in Clone 1B compared to sense control (C), which was matched by a significant reduction in average velocity (D). Data shown are mean ± SEM in A, C. A n = 3, p < 0.05 compared to Sense, C n = 20, p < 0.02 compared to Sense.