Figure 4

SM22α depletion results in increased cell migration/invasion. Despite a reduction in cell velocity REF52 cells depleted for SM22α were able to respond to a serum gradient in Dunn chemotaxis chamber assays (A). However, in invasion assays in Matrigel-coated Boyden chamber assays SM22 depleted cells exhibited a significantly enhanced capacity to invade (B; mean ± SEM 3 independent experiments, * p < 0.01 compared to sense control).