TORC1 is down regulated and Npr1p dephosphorylation is Sit4p dependent in myo1 Δ strains. Protein extracts from A) myo1Δ (40 μg), myo1Δtor1 Δ (20 μg) and B) myo1Δsit4 Δ (20 μg) mutant strains each expressing an HA-NPR1 plasmid were analyzed by Western blot. Each membrane was probed with anti-HA and anti-PGK1 antibodies. Pgk1p was used as a control. Arrows point to the expected positions of the Npr1pp-phosphorylated form and Npr1p-dephosphorylated form.