Figure 4

Inverse correlation between TORC1 and PKC1 activities. A) The PKC1 pathway was activated in wt cells upon inhibition of TORC1 with rapamycin. This pathway is constitutively activated in myo1 Δ cells. A–B) All histograms show the ratio of the intensities of each P-Slt2p band relative to the intensity of its Pgk1p loading control, averaged from duplicate experiments. Error bars represent STDError mean. B) Steady state levels of hyper phosphorylated Slt2p (P-Slt2p, 55 kDa) were assayed by Western blot using equal amounts of protein extract (50 μg) from myo1 Δ, tor1Δ, and myo1Δtor1 Δ strains treated with rapamycin (+) or with DMSO alone (-). Pgk1p was used as a loading control. C) Limiting dilution growth assay on agar medium measuring relative viability of wt, myo1Δ, tor1Δ, and myo1Δtor1 Δ strains. 10-fold dilutions are indicated at the top of the image (see Methods for details).