Dephosphorylation of eIF2α-P confirms activation of TORC1 activity in myo1Δwsc1 Δ. Western blot analysis of steady state levels of eIF2α and its phosphorylated form eIF2α-P was conducted with 50 μg per lane of whole cell protein extract derived from wt, myo1Δ, wsc1 Δ and myo1 Δwsc1 Δ strains. Pgk1p was used as a loading control. Histograms show the ratio of the relative intensities of each eIF2α band and its Pgk1p loading control, averaged from duplicate experiments. Error bars represent STD Error mean.