Figure 8

Schematic representation of the proposed regulation of TOR and Pkc1p in the myo1 Δ strain. The myo1 Δ deficiency (yellow ray) creates a cell wall stress signal that is transduced through the Wsc1p stress sensor from the Rho1p-GEF Rom2p to Rho1p which activates the PKC1 CWIP. Activation of Rho1p also leads to activation of Fks1p activity and cell wall synthesis. Regulation of cell wall integrity and actin cytoskeletal reorganization by Pkc1p could explain the synthetic rescue effect of myo1 Δ in a tor2–21^ts strain. A novel negative regulation of TORC1 by Wsc1p was observed in the myo1 Δ strain. We propose that the negative regulation of TORC1 by Wsc1p involves a novel interacting protein of Wsc1p labelled here as “X”.