Figure 1

Evaluation of the effects of xanthosine on cell morphology and growth rate of bovine MEC. A-B- Photomicrographs of hematoxylin stained monolayers of early-passage bovine MEC displaying typical cobblestone morphology of epithelial cells when cultured in xanthosine (A) or control (B) medium. Scale bar = 100 μm (photographed at 200X). C- Growth of primary MEC in the presence and absence of 200 μM xanthosine. Data, from a representative experiment using MEC at passage 3, are presented as the mean number of cells per well (± standard error) for triplicate cultures. An increase (P < 0.05) in cell number of xanthosine-treated cultures was evident on days 4 and 5. D- Bar graph depicting doubling time of MEC in xanthosine-treated and control cultures using MEC from two cows. Doubling time of xanthosine-treated cultures was consistently lower than that of control cultures regardless of the source of MEC.