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Figure 1 | BMC Cell Biology

Figure 1

From: Identification of novel mitosis regulators through data mining with human centromere/kinetochore proteins as group queries

Figure 1

Interaction between TRIP13 and p31comet. (A) GST-p31comet was expressed either alone or together with GFP-TRIP13 in asynchronous HEK293 cells. The input lysates and GST-pulldowns were probed with anti-GST and GFP antibodies. Untransfected lysates were used as controls. (B) Coomassie blue staining of SDS-PAGE gel loaded with ~0.4 μg of purified recombinant GST-p31comet and His-tagged TRIP13. (C) In vitro binding assays using recombinant His-TRIP13 at 100 nM and GST-p31comet at increasing concentrations. Proteins associated with washed nickel beads were probed with anti-6 × His and anti-GST antibodies.

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