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Figure 2 | BMC Cell Biology

Figure 2

From: Identification of novel mitosis regulators through data mining with human centromere/kinetochore proteins as group queries

Figure 2

Characterization of novel kinetochore protein TRIP13. (A) A GFP-TRIP13 transfected HeLa cell in early prometaphase was stained for centromeres (ACA, purple), BUBR1 (red) and DNA (DAPI, blue) to compare with the GFP signals (green). Images shown are maximum projection of a z-series at 1 μm interval. Bar = 10 μm.(B&C) A GFP-TRIP13 transfected prometaphase cell (B) and a cell progressing into metaphase (C) were co-stained with anti-MAD2 antibody and ACA to show GFP-TRIP13 localization only at MAD2 positive kinetochores, such as the one indicated by arrows in (C).(D) A GFP-TRIP13 transfected cell spun onto a coverslip for mitotic chromosome spread preparation was probed with DAPI and anti-HEC1 antibody. (E) A single mitotic chromosome stained with DAPI, anti-GFP and anti-HEC1 antibodies to show kinetochore localization of GFP-TRIP13.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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