Skip to main content
Figure 1 | BMC Cell Biology

Figure 1

From: A simplified but robust method for the isolation of avian and mammalian muscle satellite cells

Figure 1

Pax7 expression appears to be exclusively confined to myoblasts of horse, chicken, duck and pig. The percentage of satellite cells was determined in primary muscle cultures by immunocytochemistry for the muscle-specific transcription factor Pax7. Representative fields of equine (biceps femoris) (a), chicken (pectoralis major) (b), duck (pectoralis major) (c) and porcine (psoas major) (d) cells at an early stage of differentiation are shown. Arrows show cells which were negative for Pax7. Haematoxylin counterstaining was used. Each species analysed presented a significantly different percentage of Pax7 positive cells (p < 0.001) (e) with chicken displaying the highest number (≈90%), followed by horse (≈80%), duck (≈70%) and pig (≈40%). The results were evaluated using a two-sample t test. Graph shows the mean percentage of Pax7 positive cells with standard deviation.

Back to article page