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Figure 5 | BMC Cell Biology

Figure 5

From: Immunosuppressive activity enhances central carbon metabolism and bioenergetics in myeloid-derived suppressor cells in vitro models

Figure 5

Implication of AMPK in the maturation of BM cells to MDSCs. (A) p-AMPK to AMPK ratio in BM-derived MDSCs as analyzed by densitometry analysis of western blot bands. (B) p-AMPK and AMPK after 96 h in BM cells, BM-derived MDSCs, Comp-C pre-treated BM cells (5 μM, 15 min) cultured in the presence of GM-CSF and IL-6 (40 ng/ml). (C) Nitrate and nitrite concentrations in supernatants. Filled and empty squares (■, □) correspond to BM cells and BM-derived MDSC cultures, respectively. Empty circles (○) represent Comp-C pre-treated BM cells cultured in the presence of GM-CSF and IL-6. (D) ARG1 activity. Untreated BM cells (Black), BM cells exposed to GM-CSF and IL-6 for 24, 48, 72 or 96 h (hatched) and Comp-C pre-treated BM cells rinsed with sterile PBS and then cultured in the presence of GM-CSF and IL-6 (Grey). The same nomenclature is used for Figure 5E and F. (E, F) Ratios (referenced to the control culture) of Jurkat cell density and viability respectively.

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