Figure 4

ERL-1 expression during C. elegans development. (A) ERL-1 protein expression levels at different stages of C. elegans development were examined by Western blotting. Equal amounts of protein were loaded for each developmental stage. Western blot probed for actin shows equal protein loading of larval and adult samples. Embryos express low levels of actin relative to total amount of protein; we therefore also show a Ponceau S stained band that has equal intensity at all developmental stages. (B) Confocal images of C. elegans embryos stained with rabbit α-ERL-1 (red) and mouse α-actin (green). Scale bar = 20 μm. (C) Fluorescent micrographs of dissected gonads (gon, outlined by white dotted lines) and intestines (int, outlined by white dashed lines) stained with rabbit α-ERL-1 (red) and DAPI (blue). Scale bar = 50 μm.