Figure 7
erl-1(tm2703) has no major effect on phenotype of itr-1 mutants. The effect of erl-1(tm2703) on brood size (A), embryonic arrest (B) and defecation cycle length (C) on wild type, unc-24(e138), itr-1(sy290) unc-24(e138) and/or itr-1(sa73) was measured. sy290 is a gain-of-function and sa73 is a weak loss-of-function allele of itr-1. Because itr-1(sy290) is closely linked to unc-24(e138), the phenotype of itr-1(sy290) unc-24(e138) strains was compared to that of strains carrying unc-24(e138) alone. Black bars indicate erl-1 wild type and white bars indicate erl-1(tm2703) genotype. (A) Brood size was determined by counting the number of viable offspring per worm (n = 18-20; * indicates p-value < 0.05; ** indicates p-value < 0.01, one-way ANOVA and Newman-Keuls multiple comparison test). (B) Percentage of offspring arresting as embryos was determined. (C) Defecation cycle length was determined by measuring times between posterior body contractions (pBocs). Values represent the average (+/-SD) of six defecation cycles for each of ten worms (five worms for itr-1(sa73) strains).