Figure 8

erl-1(tm2703) does not alter response to ER-stress. (A) Wild type (wt) or erl-1(tm2703) embryos were plated onto NGM plates containing the indicated concentrations of tunicamycin (TN). After 72 hrs, animals were grouped into three categories (dead, < L4 and ≥ L4). Total number of animals scored are indicated above columns. (B) Mostly adult worms of hsp-4::GFP expressing strains (either erl-1 wt or erl-1(tm2703)) were plated onto NGM plates containing 5 μg/ml TN or DMSO only. Expression of hsp-4::GFP was determined by Western blotting using a GFP specific antibody. Western blots were also probed for ERL-1, demonstrating that ERL-1 protein levels are not altered by ER stress treatment (actin used as loading control).