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Figure 2 | BMC Cell Biology

Figure 2

From: Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling

Figure 2

Erk1/2 MAPK and Smad1/5/8 are activated in PC12 cells cultured in conditioned medium from BSO-treated hADMPCs. Western blot analysis of PC12 cells cultured in differentiation medium alone (cont), CM-BSO (−), CM-BSO (+), or differentiation medium with NGF (50 ng/mL) for 1 h. Proteins extracted from each cell culture were resolved by SDS-PAGE, transferred to a membrane, and probed with anti-phosphorylated Erk1/2 (phospho Erk1/2), anti-Erk1/2, anti-phosphorylated p38 (phospho p38), anti-p38, anti-phosphorylated Smad1/5/8 (phospho Smad1/5/8), anti-phosphorylated Akt (phospho Ark) and anti-Akt. Actin was analyzed as an internal control. Numbers below blots indicate relative band intensities as determined by the ImageJ software.

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