Activation of p38 MAPK by a constitutively active form of MKK6 resulted in elevated expression of BMP2 and FGF2. (A) A lentiviral vector expressing Flag-tagged MKK6 (glu) was transfected into hADMPCs. Expression of Flag-tagged MKK6 (glu), phosphorylated p38 MAPK and p38 MAPK was analyzed by western blotting. A CSII-EF-EGFP lentiviral vector was infected as a control (GFP). Actin was detected as an internal control. (B, C) Transcriptional levels of BMP2 (B) and FGF2 (C) were analyzed by q-PCR. The most reliable internal control gene was determined using the geNorm Software. (D, E) BMP2 (D) and FGF2 (E) secretion was analyzed by ELISA.