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Figure 5 | BMC Cell Biology

Figure 5

From: Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling

Figure 5

Activation of p38 MAPK by a constitutively active form of MKK6 resulted in elevated expression of BMP2 and FGF2. (A) A lentiviral vector expressing Flag-tagged MKK6 (glu) was transfected into hADMPCs. Expression of Flag-tagged MKK6 (glu), phosphorylated p38 MAPK and p38 MAPK was analyzed by western blotting. A CSII-EF-EGFP lentiviral vector was infected as a control (GFP). Actin was detected as an internal control. (B, C) Transcriptional levels of BMP2 (B) and FGF2 (C) were analyzed by q-PCR. The most reliable internal control gene was determined using the geNorm Software. (D, E) BMP2 (D) and FGF2 (E) secretion was analyzed by ELISA.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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