Figure 6

Transcriptional activity of NF-κB was not stimulated by oxidative stress. hADMPCs were transfected with pNF-κB-Luc and were treated with 10 μM of SB203580 or 0.1% DMSO for 2 h followed by addition of 1 mM of BSO. After 24 h, the medium was changed to fresh medium and cells were cultured for an additional 2 days. (A) The activity of NF-κB was measured by the intensity of luciferase activity driven from a minimal promoter containing tandem repeats of the NFκB transcriptional response element. Data shown represent the average of 3 independent experiments. (B) Immunocytochemical analysis against NF-κB/p65 (green). Blue staining represents nuclear staining by DAPI. Note that nuclear localization of NF-κB/p65 (white arrowhead) is only observed in hADMPCs treated with 20 ng/mL of TNF-α.