Figure 1

Effect of genistein on cell proliferation and DNA replication. (A) LM8 cells were treated for 3 days with 0–50 μM genistein, and the DNA content of the cultures was measured. *p < 0.01 (compared with the values for the untreated cultures). (B) LM8 cells were treated without (open circle) or with (filled circle) 50 μM genistein, the DNA content of the cultures was measured at the indicated intervals. *p < 0.01 (compared with the values for the untreated cultures). (C) LM8 cells were treated for 3 days without (left panel) or with (right panel) 50 μM genistein, and immunofluorescence staining of BrdU incorporated into DNA was performed. Both set of images are of the same field of view. Scale bar: 50 μm. (D) LM8 cells were treated for 3 days without (left panel) or with (right panel) 50 μM genistein and stained with trypan blue. Scale bar: 50 μm.