Figure 3

Effect of genistein on invasion, motility, MMP-2, and ALP. (A and B) LM8 cells were treated for 3 days without or with 50 μM genistein and harvested by trypsinization. Cell invasion and motility assays were performed using either matrigel-coated membranes (A) or uncoated membranes (B). *p < 0.01 (compared with the values for untreated cells). (C) LM8 cells were treated for 3 days without (left panel) or with (right panel) 50 μM genistein, and immunohistochemical staining of MMP-2 was performed. Scale bar: 50 μm. (D) LM8 cells were treated for 3 days without or with 50 μM genistein. The activity of MMP-2 secreted into conditioned media during the last 24 h of the 3-day treatment was assayed by gelatin zymography. Arrowhead shows the preform of MMP-2. (E) LM8 cells were treated for 2 days without or with 50 μM genistein, and cellular ALP activity was measured. *p < 0.01 (compared with the values for untreated cells).