Table 1 Primers used for RT-PCR analysis
Primers | Product (bp) | Cycles | |
|---|---|---|---|
Osteocalcin | 184 | 30 | |
forward | 5’-GGGCAATAAGGTAGTGAACAG-3’ | ||
reverse | 5’-GCAGCACAGGTCCTAAATAGT-3’ | ||
Drg-1 | 433 | 31 | |
forward | 5’-CGCTGAGGTGAAGCCTCTGGTG-3’ | ||
reverse | 5’-AGGGTTGTTGAGTGCGAAGCGG-3’ | ||
β-actin | 275 | 22 | |
forward | 5’-CAGGAGATGGCCACTGCCGCA-3’ | ||
reverse | 5’-AGGGTTGTTGAGTGCGAAGCGG-3’ | ||