Secreted frizzled-related protein 4 expression is positively associated with responsiveness to Cisplatin of ovarian cancer cell lines in vitro and with lower tumour grade in mucinous ovarian cancers

Table 1 Primer sequences, product size and annealing temperature

Gene	Sequence	Product size	Annealing temp
sFRP1	Forward: ATCTCTGTGCCAGCGAGTTT	202bp	55°C
sFRP1	Reverse: AAGTGGTGGCTGAGGTTGTC	202bp	55°C
sFRP2	Forward: AGGACAACGACCTTTGCATC	217bp	55°C
sFRP2	Reverse: TTGCTCTTGGTCTCCAGGAT	217bp	55°C
sFRP3	Forward: AAACTGTAGAGGGGCAAGCA	227bp	55°C
sFRP3	Reverse: GGCAGCCAGAGCTGGTATAG	227bp	55°C
sFRP4	Forward: CGATCGGTGCAAGTGTAAAA	181bp	54°C
sFRP4	Reverse: GACTTGAGTTCGAGGGATGG	181bp	54°C
sFRP5	Forward: GATGTGCTCCAGTGACTTTG	352bp	60°C
sFRP5	Reverse: GCAGGGGTAGGAGAACATGA	352bp	60°C
GAPDH	Forward: CAGAACATCATCCCTGCATCCACT	185bp	59°C
GAPDH	Reverse: GTTGCTGTTGAAGTCACAGGAGAC	185bp	59°C
L19	Forward: GGACAGAGTCTTGATGATCTC	194bp	51°C
L19	Reverse: CTGAAGGTCAAAGGGAATGTG	194bp	51°C
β-actin	Forward: GCACCAAGGATGGAGATGTT	173bp	55°C
	Reverse: GGACAGAGTCTTGATGATCTC

ISSN: 2661-8850