q-PCR of microarray data reveals miRNAs involved in stress response and associated with Dys and Dg signaling. q-PCR verification of microarray screen reveals miRNAs that fall into each functional category. (a) miR-124 and miR-iab-4-5p levels do not go down in Dys and Dg mutants when high temperature stress is applied. (b) miR-959 was found to be a general temperature stress response miRNA. (c) miR-962 and miR-975 have reduced levels in response to loss of Dg alone. (d) miR-956, miR-252 and miR-980 all are downregulated in response to loss of Dg, Dys and Syn1. (e) Relative levels of Dg mRNA in Dys and dsSyn1 mutants, Dys mRNA levels in Dg and dsSyn1 mutants and relative levels of Syn1 mRNA in Dys, Dg and dsSyn1 mutants. Each comparison is made to the closest control genotype (Oregon R or tub-Gal4>+) to the left of the test genotype. Statistics were done using a one-tailed Student’s t-test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n.s – not significant. (f) Venn diagram shows overlap among verified miRNAs and the relevant functional groups.