Figure 4

AmpA influences cell substrate adhesion and substrate contact area.A) AmpA influences cell substrate adhesion. Cells grown on petridishes were shaken at increasing shaker speeds, RPM (rotations perminute) for 45 minutes and the percent of cells remaining on the dishwas determined. B) AmpA influences the % of the cell surface incontact with the substrate. To determine the percentage of the cell incontact with the substrate cells were imaged using transmitted light andin reflection mode to identify the area of the cell in contact with thesubstrate (Additional file 7 for raw data).The area of the cell and the area of the contact sites were determinedand the contact sites were expressed as a % of the total area of thecell. The number of cells imaged was 65 to 125 for cells sitting onglass and 50 to 95 for cells migrating under agar. Results are averagesfrom two different experiments preformed on different days. Error barsare the standard error of the mean. * indicates a significant differencewith a P value < 0.05 using a two tailed paired Student’st-test.