The adhesion modulation protein, AmpA localizes to an endocytic compartment andinfluences substrate adhesion, actin polymerization and endocytosis invegetative Dictyostelium cells

Table 1 Comparison of plaque size on rich broth and minimal media plates

Strain	Plaque area (mm²) on minimal medium plates	Plaque area (mm²) on rich broth plates
WT	1.41 +/−0. 17	0.94+/−0.08
KO	0.28 +/− 0.04*	0.020+/−0.002*
OE	2.33 +/− 0.28*	0.018+/−0.002*

Cells were plated for 4 days on LP (minimal media plates) withE.coli B/r and for 11 days on 1/2xHL5 plates onE.coli B/r (rich broth plates). The areas of between 30to 70 plaques per strain were determined on a minimum of 5 differentplates per strain. Experiments were repeated at least 3 times withsimilar results on different days. Absolute plaque size differs withdifferent batches of plates but the relative plaque size differencesbetween strains are highly reproducible. Errors are standard errorof the mean. * indicates difference is significant from Wt (p value< 0.001). There was no significant difference in plaque sizebetween KO and OE (p value = 0.26) on rich broth plates butsignificant differences between KO and OE on minimal media plates (pvalue < 0.001).

ISSN: 2661-8850