Figure 1

Representative images of porcine chromosome territories in nuclei derived from embryonic and adult fibroblasts, mesenchymal stem cells (MSCs) and lymphocytes. Chromosome territories are delineated with biotinylated whole chromosome painting probes amplified by degenerate oligonucleotide primed PCR (DOP-PCR) and visualised via strepavidin conjugated to cyanine 3 (red), DNA synthesis in S-phase nuclei is revealed by BrdU incorporation and indirect immunofluorescent detection with an antibody conjugated to FITC (green) and the DNA within the interphase nuclei stained with the DNA intercalator dye DAPI (blue). The lymphocyte image depicts dual-coloured FISH with the single Y chromosome labelled with digoxigenin and visualised by an anti-dig antibody conjugated to FITC. Bar, 10 μm.