Figure 1

N6L inhibits the in vitro adhesion, proliferation, migration and motility of HUVECs. (A) Inhibition of HUVEC cell adhesion by N6L. An equal number of HUVECs were incubated with increasing concentrations of N6L for 30 min before seeding. After a 45 min incubation period, adherent cells were measured by the crystal violet assay. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments (B). Inhibition of HUVECs proliferation by N6L. Cells were cultured for 3 days in the presence of increasing concentrations of N6L. Cell proliferation was quantified by crystal violet staining. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments. (C) Migration of cells through Transwell filters. The lower compartment of Transwell filters (8 μm pores) was filled with growth media containing 0.5% BSA, and increasing concentrations of N6L. An equal number of HUVECs was resuspended in a growth medium containing 0.5% BSA, and transferred into Transwell inserts. Cells that successfully migrated through the filter pores, were fixed, stained and quantified by counting the entire area of each filter. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments. (D) Confluent cell monolayers were scratched and cells were left to heal the wound in the presence of increasing concentrations of N6L. 48 h later the plates were photographed. The dashed lines indicate the remaining wound.