SNF8 enhances TRPC6-mediated currents. Whole-cell currents were measured in HEK cells expressing the M1 receptor and transiently transfected with either wild-type TRPC6 (A) or E897K mutant TRPC6 (B), alone (black line) or with SNF8 (red). Cells were stimulated with 100 μM carbachol to activate TRPC6-mediated current. C. Average normalized current density in response to carbachol. One way ANOVA Fisher LSD; ** p value <0.01 vs. TRPC6 WT, ## p value <0.01 between SNF8 and non-SNF8 expressing cells; n=6.