Detergent-resistant membrane preparation. M1R cells stably expressing TRPC6 were transiently transfected with control vector (A) or HA-SNF8 expression construct (B). Triton X-100 detergent resistant membranes were separated from other proteins by sucrose gradient centrifugation, and the abundance of FLAG-TRPC6 and HA-SNF8 in each fraction analyzed by Western blot. Caveolin-1 represents a marker of lipid rafts. Fraction 1 represents the top of the gradient.