Effect of ANG-II and ET-1 in cardiac myocytes. Effect of neurohumoral factors on ob, ob-Ra, and ob-Rb mRNA expression in neonatal rat cardiac myocytes. Neonatal rat cardiac myocytes were treated with ANGII (1 to 10 μM), ET-1 (0.01 to 0.1 μM) for 24 h. All quantitative PCR assay data for ob, ob-Ra, and ob-Rb mRNA is normalized by GAPDH mRNA of the same sample, and expressed as fold increase compared with the mean level of control dishes. n=4 dishes in each group. Values are reported as means ± SD. *p<0.05 vs. control.