Effect of cyclic mechanical stretch in cardiac myocytes. Effect of cyclic mechanical stretch on ob, ob-Ra, and ob-Rb mRNA expression in neonatal rat cardiac myocytes. Neonatal rat cardiac myocytes were treated with mechanical stretch for 1 to 24 h. All quantitative PCR assay data for ob, ob-Ra, and ob-Rb mRNA is normalized by GAPDH mRNA of the same sample, and expressed as fold increase compared with the mean level of control wells. n=4 dishes in each group. Values are reported as means ± SD. *p<0.05 vs. control.