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Figure 1 | BMC Cell Biology

Figure 1

From: Rab11-FIP3 is a cell cycle-regulated phosphoprotein

Figure 1

FIP3 translocates from cytosol to membranes during the cell cycle Panels A and B. Synchronized HeLa cells were harvested at different time points after release from the nocodazole block as shown. Cells were fractionated into cytosolic and membrane fractions and levels of FIP3 analyzed by immunoblotting, with 60 μg of each fraction loaded in each lane. Data shown in panel A are the means and standard deviations from three independent experiments. In panel B, "prophase/metaphase" represents samples harvested at 0 min after release from nocodazole block, while "telophase" represents samples harvested 70 min after release from nocodazole block. Transferrin Receptor (TfR) was used as a marker for membranes, and RCP/FIP1 as an unrelated FIP for comparative purposes.

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