Figure 4

Palmitoylation stabilizes Gαi2 coupling. (a) The FRET between CFPOPRM1 and YFPGαi2 and the FRET between CFPC170A and YFPGαi2 were determined in HEKOPRM1 and HEKC170A cells. (b-c) Colocalization between FLAGOPRM1 and Gαi2 (or FLAGC170A and Gαi2) was compared in HEKOPRM1 and HEKC170A cells after transfection (b). The amounts of Gαi2 precipitated with FLAGOPRM1 and FLAGC170A in the two cell lines were compared in (c). The amount of Gαi2 precipitated with FLAGOPRM1 in HEKOPRM1 cells was used for normalization. One-way ANOVA with Dunnett's test as a post-hoc test was used. The error bars and "*" represent the standard deviations and significant changes (p < 0.05, n > 3), respectively.