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Table 2 Palmitoylation impairs morphine-induced receptor signaling.

From: Palmitoylation and membrane cholesterol stabilize μ-opioid receptor homodimerization and G protein coupling

  

HEKOPRM1

HEKOPRM1

+2-BP

HEKC170A

Morphine-Induced Signaling

   

   AC Inhibition

K I (nM)

9.3 ± 0.89

15 ± 1.2*

14 ± 1.3*

 

Max. Inh. (%)

84 ± 2.8

56 ± 5.1*

63 ± 4.3*

   pERK

(% of basal)

239 ± 10

154 ± 10*

164 ± 12*

  1. The percentage inhibition of forskolin-induced cAMP increase by various concentrations of morphine was analyzed with GraphPad Prism 5.0. The results are presented as the maximum inhibition (Max. Inh.) and the IC50 values (KI). ERK phosphorylation was indicated by the amount of phosphorylated ERK after 5 min of treatment with 1 μM morphine (see blots provided in Figure 1g). The amount of total ERK and the results under control conditions were used for normalization. Experiments were repeated at least four times. Data were analyzed by one-way ANOVA with post-hoc Dunnett's test. Standard deviations are provided, and "*" represents significant changes (p < 0.05).
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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