Figure 4

IFN-α-induced loss of mitochondrial membrane potential and apoptosis are partially abrogated in dnPKC-δ-expressing cells, while somewhat enhanced in caPKC-δ-expressing cells. Daudi cells were transfected with Flag-dnPKC-δ, Flag-caPKC-δ, or the control vector alone, and incubated with selection media containing hygromycin B, followed by limiting dilution to obtain clone(s). The resulting clones were confirmed to express Flag-dnPKC-δ or Flag-caPKC-δ (A). These clones were stimulated with 1,000 U/ml IFN-α for the indicated time periods, followed by assays for mitochondrial membrane potential (B, 48 h-incubation) and PI positive cells (C). Arrow indicates specific band for PKC-δ. †, non-specific band. The results from three independent experiments (B&C) are represented as mean ± SD. *Significantly different (p < 0.05) from control vector alone.