Figure 5
From: PKC-δ mediates interferon-α-induced apoptosis through c-Jun NH2-terminal kinase activation
IFN-α-induced activation of JNK1 and TRAIL promoter is partially reduced in dnPKC-δ-expressing cells. The dnPKC-δ-expressing Daudi cells were stimulated with 1,000 U/ml IFN-α for 6 h, and the levels of phospho-JNK (A), TRAIL promoter activity (B), and TRAIL protein expression on the cell surface (C) were determined by Western blotting, the TRAIL-Luc reporter assay, and flow cytometry, respectively. MFI represents mean fluorescent intensity. The results from three independent experiments (B) are represented as mean ± SD. *Significantly different (p < 0.05) from control vector alone.