Figure 4

DNp73 over expression dramatically increases Nanog promoter driven luciferase gene expression level. While, on the contrary, p53 and TAp73 over expression significantly suppress Nanog promoter driven luciferase gene expression. The transfection construct amounts were applied at 10 ng or 500 ng per well of 24 well plates respectively. The p53 vector was served as positive control and the results were compared to treatment with vector only. Error bars: s.d. n = 3 independent experiments.