Figure 4From: Analysis of Sec61p and Ssh1p interactions in the ER membrane using the split-ubiquitin systemPrey constructs for the split ubiquitin assay. Proteins were fused as indicated to NubG. Topology of the fusion proteins in the ER membrane is shown. Interaction of Wbp1-CPLV with Ost1-Nub was used as positive control. Expression of Nub-Alg5 in the ER membrane was used as negative control for all bait constructs.Back to article page