Figure 4
From: Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa
Comparison of PPP1R2 and PPPR2P3 phosphorylation by GSK3 and CK2. Recombinant PPP1R2 and PPP1R2P3 were incubated in kinase buffer with reactive ATP (0); or in the presence of GSK3β kinase (G), CK2 kinase (C) or both kinases (G/C), for 90 min at 30oC. Protein phosphorylation was detected by autoradiography (32P) following SDS-PAGE. Immunoreactivity detected with a specific sheep anti-PPP1R2 antibody is shown for comparative purposes.