Figure 5
From: Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa
PPP1R2/PPP1R2P3 are present in human testis and ejaculated sperm. (A), A Western blot of human testis and sperm screening (50 μg) of PPP1R2/PPP1R2P3 and PPP1CC isoforms was performed using sheep anti-PPP1R2 and CBC3C antibodies. Extracts were prepared in 1%SDS. β-Tubulin was used as loading control. (B), PPP1R2/PPP1R2P3 was immunoprecipitated from a human sperm heat-stable extract with sheep anti-PPP1R2 antibody followed by Western blotting with the same antibody. Recombinant His-PPP1R2P3 (50ηg) and sperm lysate before boiling (soluble fraction) were used as controls. PPP1R2 was immunoprecipitated using dynabeads protein G. IP, immunoprecipitation.