Table 1 Peptides identified by Orbitrap Velos mass spectrometry for PPP1R2, PPP1R2P3 or both and for PPP1CC2, after immunoprecipitation of human sperm samples with sheep or rabbit anti-PPP1R2 antibodies. aa, amino acids; pI, isoelectric point
From: Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa
Protein name | Uniprot ID | MW (Da) | pI | Protein size (aa) | Coverage | Mascot score |
|---|---|---|---|---|---|---|
PPP1R2 | IPP2_HUMAN | 23.015 | 4.64 | 205 | 22.0% | 74.12 |
Peptide | Range (start-end) | Number of spectra | m/z meas. | z | Mascot score | |
K.WDEMNILATYHPADKDYGLMK.I | 47–67 | 3 | 848.39 | 3 | 47.40 | |
K.TSTTSSMVASAEQPRGNVDEELSK.K | 19–42 | 2 | 847.40 | 3 | 26.72 | |
Protein Name | Uniprot ID | MW (Da) | pI | Protein size (aa) | Coverage | Mascot score |
PPP1R2P3 | IPP2M_HUMAN | 23.048 | 4.82 | 205 | 7.3% | 48.95 |
Peptide | Range (start-end) | Number of spectra | m/z meas. | z | Mascot score | |
K.WDEINILATYHPADK.G | 47–61 | 3 | 595.96 | 3 | 48.95 | |
Protein Name | MW (Da) | pI | Protein size (aa) | Coverage | Mascot score | |
Common to both PPP1R2 and PPP1R2P3 | 49.3% | 546.34 | ||||
Peptide | Range (start-end) | Number of spectra | m/z meas. | z | Mascot score | |
R.KLAAAEGLEPK.Y | 103–113 | 7 | 563.83 | 2 | 70.38 | |
R.IQEQESSGEEDSDLSPEER.E | 116–134 | 5 | 1082.46 | 2 | 117.82 | |
K.LAAAEGLEPK.Y | 104–113 | 5 | 499.78 | 2 | 63.21 | |
K.LHYNEGLNIK.L | 146–155 | 4 | 400.88 | 3 | 53.17 | |
R.KLHYNEGLNIK.L | 145–155 | 4 | 664.87 | 2 | 51.25 | |
K.LHYNEGLNIKLAR.Q | 146–158 | 2 | 514.29 | 3 | 23.57 | |
R.IQEQESSGEEDSDLSPEEREK.K | 116–136 | 2 | 807.69 | 3 | 59.32 | |
K.DLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNK.L | 164–201 | 1 | 1434.23 | 3 | 30.63 | |
K.YRIQEQESSGEEDSDLSPEER.E | 114–134 | 1 | 828.70 | 3 | 11.42 | |
K.LAAAEGLEPKYR.I | 104–115 | 1 | 439.91 | 3 | 15.97 | |
K.TSTTSSMVASAEQPR.G | 19–33 | 1 | 784.86 | 2 | 49.60 | |
Protein Name | Uniprot ID | MW (Da) | pI | Protein size (aa) | Coverage | Mascot score |
PPP1CC2 | PP1G_HUMAN | 38.518 | 5.78 | 337 | 15% | 252.57 |
Peptide | Range (start-end) | Number of spectra | m/z meas. | z | Mascot score | |
R.VASGLNPSIQK.A | 315–325 | 5 | 557.32 | 2 | 54.76 | |
R.GVSFTFGAEVVAK.F | 222–234 | 2 | 656.35 | 2 | 72.94 | |
K.NVQLQENEIR.G | 27–36 | 2 | 621.83 | 2 | 42.11 | |
K.YPENFFLLR.G | 114–122 | 1 | 599.82 | 2 | 39.6 | |
K.LNIDSIIQR.L | 7–15 | 1 | 536.31 | 2 | 43.16 | |