Figure 1
From: High-throughput fluorescent-based NKCC functional assay in adherent epithelial cells
A) Immunofluorescence confocal analysis of Na-K-ATPase (red signal) and c-NKCC2 (green signal) in c-NKCC2-transfected LLC-PK1 cells grown on coverslips. The merged signal of planar xy and xz projections was depicted. Na-K-ATPase and c-NKCC2 are selectively expressed at the basolateral and apical membrane respectively. B) Immunofluorescence confocal analysis of Na-K-ATPase (red signal) and the endogenous NKCC2, (green signal) in untransfected LLC-PK1 cells grown on coverslips. The merged signal of planar xy and xz projections was depicted. Na-K-ATPase was selectively expressed at the basolateral membrane whereas the endogenous NKCC2 was absent. Inset: Immunolocalization of the endogenous NKCC2 in a rat kidney section.